COMPREHENSIVE 3-MONTH LONGEVITY OPTIMIZATION PROTOCOL

THE BIOHACKER'S BLUEPRINT v3.0

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TABLE OF CONTENTS

1. Foundation & Baseline Testing
2. Month 1: Foundation & Calibration
3. Month 2: Optimization & Intensification
4. Month 3: Integration & Peak Performance
5. Supplement Stacks & Cycling Protocols
6. Dietary Protocols
7. Exercise Programming
8. Sleep Architecture Optimization
9. Stress Resilience & Cognitive Enhancement
10. Tracking, Wearables & Biomarker Monitoring
11. Environmental & Lifestyle Optimization
12. Weekly Schedules & Daily Templates

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PHASE 0: PRE-PROTOCOL BASELINE (Week 0 — Before Day 1)

Comprehensive Lab Panel

Blood Biomarkers (Request from physician or use services like InsideTracker, Marek Health, or Function Health)

Metabolic Panel:

• Fasting glucose, fasting insulin, HbA1c, HOMA-IR calculation
• Complete lipid panel: Total cholesterol, LDL-C, LDL-P (particle number), HDL-C, HDL-P, triglycerides, VLDL, Lp(a), ApoB, ApoA1, oxidized LDL
• Comprehensive metabolic panel (CMP)

Hormonal Panel:

• Total & free testosterone, SHBG, estradiol (sensitive assay)
• DHEA-S, pregnenolone, cortisol (AM draw at 8 AM)
• Free T3, free T4, TSH, reverse T3, thyroid antibodies (TPO, TG)
• IGF-1, growth hormone (optional stimulation test)
• Prolactin

Inflammatory Markers:

• hs-CRP, homocysteine, fibrinogen
• ESR, ferritin (also iron marker), IL-6 (if available)

Nutrient Status:

• 25-OH Vitamin D, RBC magnesium, zinc (serum + RBC), copper
• Vitamin B12, folate (serum + RBC), iron panel (serum iron, TIBC, ferritin, transferrin saturation)
• Omega-3 Index (OmegaQuant test)

Organ Function & Aging:

• CBC with differential
• Comprehensive metabolic panel (liver: ALT, AST, GGT, ALP; kidney: BUN, creatinine, eGFR, cystatin C)
• Uric acid

Advanced Longevity Markers (optional but recommended):

• Biological age testing: TruAge (epigenetic clock), GlycanAge
• Telomere length (e.g., via SpectraCell or Life Length)
• NAD+ levels (Jinfiniti IntracellularNAD test)
• 8-OHdG (oxidative DNA damage marker, urine)

Functional Assessments

• DEXA scan (body composition, visceral fat, bone density)
• VO2max test (gold standard cardiorespiratory fitness)
• Grip strength (both hands, using dynamometer)
• Resting heart rate, HRV baseline (7-day average)
• Blood pressure (3 readings averaged, both arms)
• Continuous glucose monitor (CGM) — 14-day baseline (Abbott Libre 3 or Dexcom G7)
• Cognitive baseline: Cambridge Brain Sciences or BrainHQ assessment
• Sleep baseline: 7 nights with Oura Ring or WHOOP

Genetic Testing (One-time)

• Whole genome sequencing or SNP panel (23andMe + upload to SelfDecode, Genetic Lifehacks, or Promethease)
• Key SNPs to review: MTHFR, COMT, APOE, CLOCK genes, ACTN3, CYP enzymes, SOD2, BDNF Val66Met

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MONTH 1: FOUNDATION & CALIBRATION (Weeks 1–4)

Theme: "Build the Base"

Primary Goals: Establish circadian rhythm mastery, gut optimization, metabolic flexibility, movement foundation, stress resilience baseline

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1.1 SUPPLEMENT STACK — MONTH 1: "Foundation Stack"

Morning Stack (taken with breakfast, ~7:00 AM)

Supplement	Dosage	Purpose	Notes
NMN (nicotinamide mononucleotide)	500 mg sublingual	NAD+ precursor, cellular energy	Take sublingual for better absorption; Renue By Science or ProHealth brand
Trans-Resveratrol	500 mg	Sirtuin activation, synergist with NMN	Take with fat source (yogurt/olive oil); Micro-encapsulated form preferred
Vitamin D3	5,000–10,000 IU (dose based on baseline levels)	Immune, bone, gene expression	Target serum 60–80 ng/mL; take with K2 and fat
Vitamin K2 (MK-7)	200 mcg	Calcium metabolism, synergist with D3	Prevents arterial calcification
Omega-3 (EPA/DHA)	2g EPA / 1g DHA	Anti-inflammatory, membrane fluidity, brain	Molecular-distilled; Nordic Naturals or Carlson; target Omega-3 Index >8%
Magnesium L-Threonate	1,000 mg (144 mg elemental Mg)	Brain magnesium, cognitive function	Crosses BBB; Magtein brand
B-Complex (methylated)	1 capsule	Methylation support, energy	Must contain methylfolate + methylcobalamin; Thorne Basic B or Seeking Health
Creatine Monohydrate	5g	Cognitive + muscular performance, neuroprotection	Creapure brand; mix in water/shake; take daily, no cycling needed
Vitamin C (liposomal)	1,000 mg	Antioxidant, collagen synthesis, immune	Liposomal for absorption; LivOn Labs

Midday Stack (taken with lunch, ~12:30 PM)

Supplement	Dosage	Purpose	Notes
Sulforaphane (or broccoli sprout extract)	10–20 mg sulforaphane equivalent	Nrf2 activation, detox pathways	Avmacol or BROQ brands; or grow broccoli sprouts
Quercetin Phytosome	500 mg	Senolytic (mild), anti-inflammatory	Thorne Quercetin Phytosome for absorption
CoQ10 (Ubiquinol form)	200 mg	Mitochondrial function, heart health	Kaneka ubiquinol; take with fat
Alpha-Lipoic Acid (R-ALA)	300 mg	Universal antioxidant, glucose metabolism	R-form only; take on empty stomach if tolerated

Evening Stack (taken with dinner or 2 hours before bed, ~6:30 PM)

Supplement	Dosage	Purpose	Notes
Magnesium Glycinate	400 mg (before bed)	Sleep, muscle relaxation, stress	In addition to morning threonate; different forms serve different purposes
Apigenin	50 mg	Sleep quality, CD38 inhibition (protects NAD+)	Found in chamomile; Andrew Huberman protocol
L-Theanine	200 mg	GABA modulation, calm focus, sleep onset	Only if needed; some people are non-responders
Tart Cherry Extract	500 mg	Natural melatonin source, recovery	Preferred over synthetic melatonin for Month 1

Cycling Notes — Month 1

• All supplements taken daily for the full month to establish baseline response
• Track subjective response in a daily journal (1–10 energy, mood, sleep quality, cognition)
• If any GI distress, reduce omega-3 and ALA first, reintroduce gradually

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1.2 DIETARY PROTOCOL — MONTH 1: "Metabolic Reset"

Protocol: Modified Mediterranean-Ketogenic Hybrid + Time-Restricted Eating (TRE)

Eating Window: 16:8 intermittent fasting

• Feeding window: 11:00 AM – 7:00 PM (8 hours)
• First meal: 11:00 AM
• Last meal finished by: 7:00 PM
• Fasting window: 7:00 PM – 11:00 AM (16 hours)

During fasting window (allowed):

• Water (with electrolytes: ¼ tsp salt, ¼ tsp potassium chloride per liter)
• Black coffee (before 2 PM only)
• Plain green tea / matcha
• Mineral water with lemon

Macronutrient Targets (adjust based on DEXA and activity level)

Training Days (4 days/week):

• Calories: TDEE (maintenance) — approximately 2,200–2,600 kcal for active male, adjust for female
• Protein: 1.6–2.2 g/kg lean body mass (e.g., 130–170g for 80kg male with 75kg LBM)
• Fat: 30–35% of calories (primarily monounsaturated + omega-3)
• Carbs: 100–150g (timed around training)
• Fiber: 35–50g minimum

Rest Days (3 days/week):

• Calories: TDEE minus 10–15%
• Protein: Same as training days (maintain)
• Fat: 35–40% of calories
• Carbs: 50–80g (lower carb, bordering ketogenic)
• Fiber: 35–50g minimum

Food Quality Framework

Protein Sources (prioritize):

• Wild-caught salmon, sardines, mackerel (3x/week minimum)
• Pasture-raised eggs (whole, 2–4/day)
• Grass-fed beef/bison (2x/week)
• Organic chicken thighs
• Wild game (venison, elk) when available
• Bone broth (homemade, daily — 1 cup, rich in glycine/collagen)
• Whey protein isolate (grass-fed) or plant protein for shakes

Fat Sources:

• Extra virgin olive oil (high-polyphenol, >500 mg/kg polyphenols; 2–4 tbsp/day)
• Avocados (1/2–1 daily)
• Raw nuts: macadamia, walnuts, almonds (1 oz/day)
• MCT oil (1 tbsp in morning coffee on rest days for mild ketosis)
• Ghee or grass-fed butter (cooking)

Carbohydrate Sources (emphasize low-glycemic, high-fiber):

• Sweet potatoes, purple potatoes
• Wild rice, quinoa
• Legumes: lentils, black beans (if tolerated — excellent for microbiome)
• All berries: blueberries, blackberries, raspberries, strawberries (1 cup/day)
• Cruciferous vegetables: broccoli, cauliflower, Brussels sprouts, kale (2+ cups/day)
• Leafy greens: spinach, arugula, Swiss chard (2+ cups/day)
• Fermented foods: sauerkraut, kimchi, kefir (1 serving/day minimum)

Foods to Eliminate Completely:

• Refined seed/vegetable oils (soybean, canola, sunflower, safflower, corn oil)
• Added sugars and artificial sweeteners (stevia and monk fruit OK sparingly)
• Ultra-processed foods (anything with >5 unrecognizable ingredients)
• Trans fats
• Excessive alcohol (limit to 2 drinks/week maximum; red wine preferred)
• Conventional dairy (replace with grass-fed/A2 or eliminate if sensitive)

Specific Daily Meal Templates — Month 1

TRAINING DAY EXAMPLE:

Meal 1 (11:00 AM — Break-fast):

• 3 pasture-raised eggs scrambled in ghee
• 1/2 avocado
• 1 cup sautéed spinach + mushrooms
• 1/4 cup sauerkraut
• 1 cup bone broth on the side
• Calories: ~550 | P: 30g | F: 40g | C: 12g

Meal 2 (2:30 PM — Pre-training fuel, eaten 90 min before workout):

• 6 oz wild-caught salmon
• 1 medium sweet potato with 1 tbsp EVOO
• Large mixed green salad with arugula, walnuts, blueberries, balsamic
• Calories: ~650 | P: 42g | F: 28g | C: 55g

Post-Workout Shake (5:00 PM):

• 30g grass-fed whey isolate
• 1 cup frozen mixed berries
• 1 tbsp almond butter
• 5g creatine (if not taken AM)
• Water/unsweetened almond milk
• Calories: ~300 | P: 35g | F: 10g | C: 25g

Meal 3 (6:30 PM — Dinner):

• 6 oz grass-fed beef or bison
• 2 cups roasted cruciferous vegetables (broccoli, Brussels sprouts) with EVOO
• 1/2 cup cooked lentils or quinoa
• 1 cup kimchi
• Calories: ~600 | P: 45g | F: 25g | C: 45g

Daily Totals: ~2,100 kcal | P: 152g | F: 103g | C: 137g (Adjust portions up/down based on individual needs)

REST DAY EXAMPLE:

Meal 1 (11:00 AM):

• Smoothie: 1 cup full-fat kefir, 1/2 cup blueberries, 1 tbsp MCT oil, 30g collagen peptides, handful spinach, 1 tbsp cacao nibs
• Calories: ~450 | P: 35g | F: 25g | C: 22g

Meal 2 (2:30 PM):

• Large salad: mixed greens, 5 oz grilled chicken, 1/2 avocado, pumpkin seeds, EVOO + lemon dressing, 1/4 cup fermented vegetables
• Calories: ~550 | P: 42g | F: 35g | C: 15g

Meal 3 (6:30 PM):

• 6 oz wild sardines (canned in EVOO)
• Roasted cauliflower "steaks" with turmeric and black pepper
• Side of mixed roasted vegetables
• 1 cup bone broth
• Calories: ~500 | P: 38g | F: 30g | C: 20g

Daily Totals: ~1,500 kcal | P: 115g | F: 90g | C: 57g

Week 4 Special Protocol: 72-Hour Fasting-Mimicking Diet (FMD)

During the last 3 days of Month 1 (Days 26–28), implement a Prolon-style FMD:

Day	Calories	Composition
Day 1	1,100 kcal	10% protein, 56% fat, 34% carb
Day 2	800 kcal	9% protein, 44% fat, 47% carb
Day 3	800 kcal	9% protein, 44% fat, 47% carb

Allowed foods during FMD: Vegetable soups, olives, small portions of nuts, herbal teas, small amounts of kale chips, flax crackers, 1 tbsp EVOO per meal. This triggers autophagy, stem cell regeneration, and metabolic reset without full water fasting.

Refeeding (Day 29): Break with bone broth, then small meal of easily digestible foods. Gradually resume normal eating over 24 hours.

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1.3 EXERCISE PROGRAMMING — MONTH 1: "Build the Engine"

Weekly Structure

Day	Focus	Duration	Intensity
Monday	Strength A — Upper Push/Pull	55 min	Moderate-High
Tuesday	Zone 2 Cardio	45–60 min	Low (conversational)
Wednesday	Strength B — Lower Body + Core	55 min	Moderate-High
Thursday	Active Recovery + Mobility	30–40 min	Low
Friday	Strength C — Full Body + Power	55 min	Moderate-High
Saturday	Zone 2 Cardio + Zone 5 Finisher	50–65 min	Low + High
Sunday	Full Rest or Gentle Walk (nature)	30–60 min	Very Low

Detailed Workout Protocols

MONDAY — Strength A: Upper Push/Pull

Warm-up (10 min):

• Cat-cow x 10
• Band pull-aparts x 15
• Shoulder CARs (Controlled Articular Rotations) x 5 each direction
• Push-up to downward dog x 8
• Light rowing machine 3 min

Main Work (40 min):

Exercise	Sets x Reps	Tempo	Rest	Notes
Barbell Bench Press	4 x 6–8	3-1-1-0	2.5 min	RPE 7–8; progressive overload weekly
Weighted Pull-ups (or Lat Pulldown)	4 x 6–8	3-1-1-0	2.5 min	Add weight when 4x8 achieved
Seated DB Overhead Press	3 x 8–10	2-1-1-0	2 min
Cable Row (close grip)	3 x 10–12	2-1-2-0	90 sec	Squeeze at contraction
Dips (weighted if possible)	3 x 8–12	2-0-1-0	90 sec
Face Pulls	3 x 15–20	2-1-2-0	60 sec	Posterior delt health

Finisher:

• Farmer's carries: 3 x 40m with heavy dumbbells (grip strength = longevity predictor)

Cool-down (5 min):

• Pec doorway stretch 30 sec/side
• Lat hang from pull-up bar 30 sec x 2
• Deep breathing: 5 breaths, 4-7-8 pattern

WEDNESDAY — Strength B: Lower Body + Core

Warm-up (10 min):

• Foam roll quads, glutes, adductors (2 min)
• 90/90 hip switches x 10
• Goblet squat hold 30 sec
• Walking lunges x 10/side
• Glute bridges x 15

Main Work (40 min):

Exercise	Sets x Reps	Tempo	Rest	Notes
Back Squat (or Front Squat)	4 x 6–8	3-1-1-0	3 min	Depth: hip crease below knee
Romanian Deadlift	4 x 8–10	3-1-1-0	2.5 min	Hamstring focus; hip hinge mastery
Bulgarian Split Squat	3 x 10/leg	2-1-1-0	90 sec/leg	Hold DBs; balance + unilateral strength
Leg Curl (Nordic if possible)	3 x 8–12	3-0-1-0	90 sec	Nordic curl eccentrics if gym allows
Pallof Press	3 x 12/side	2-2-2-0	60 sec	Anti-rotation core stability
Dead Bug	3 x 10/side	Controlled	60 sec	Full exhale at extension
Calf Raises (standing)	4 x 12–15	2-2-1-0	60 sec	Full ROM; pause at bottom stretch

Finisher:

• Wall sit: 2 x max hold (target 90 sec)

FRIDAY — Strength C: Full Body + Power

Warm-up (10 min):

• Jump rope 2 min
• Arm circles, leg swings
• Medicine ball slams x 10
• Bodyweight squats x 10

Main Work (40 min):

Exercise	Sets x Reps	Tempo	Rest	Notes
Trap Bar Deadlift	4 x 5–6	Explosive up, 3 sec down	3 min	Heavy; RPE 8
Barbell Push Press	3 x 6–8	Explosive	2.5 min	Power development
Weighted Chin-ups	3 x 6–8	2-1-1-0	2.5 min
DB Walking Lunges	3 x 10/leg	2-0-1-0	2 min
Cable Woodchops	3 x 12/side	Controlled	60 sec	Rotational power
KB Swings	3 x 15	Explosive	90 sec	Hip power; cardiovascular
Hanging Leg Raises	3 x 10–15	Controlled	60 sec	Grip + core

Cardio Protocols — Month 1

ZONE 2 SESSIONS (Tuesday + Saturday):

What is Zone 2? Heart rate at which you can maintain nasal breathing and hold a conversation. Approximately 60–70% of max HR. For most people: 120–150 bpm depending on age and fitness.

Calculate your Zone 2:

• MAF Method: 180 – age = top of Zone 2 (e.g., 180 – 35 = 145 bpm)
• Adjust: subtract 5 if recovering from illness/injury; add 5 if consistently training 2+ years

Modalities (choose one per session; vary for engagement):

• Incline treadmill walking (10–15% grade, 3.0–3.5 mph) — excellent for Zone 2
• Cycling (stationary or outdoor)
• Rowing machine (excellent full-body Zone 2)
• Swimming
• Rucking (20–30 lb pack, outdoor walk)

Duration: 45–60 minutes Frequency: Minimum 150 minutes Zone 2 per week (Peter Attia protocol)

Why: Zone 2 training improves mitochondrial density, fat oxidation, metabolic flexibility, and is the single most impactful exercise modality for longevity according to current evidence.

SATURDAY ZONE 5 FINISHER (after Zone 2 session):

• 4 x 30-second all-out sprints (bike or rower preferred for joint safety)
• 4 minutes easy recovery between sprints
• Total additional time: ~20 minutes
• This trains VO2max, the strongest predictor of all-cause mortality

Movement & Mobility (Thursday — Active Recovery)

30–40 minute session:

• Yoga flow (Ashtanga primary series modified, or follow Yoga with Adriene "flexibility" sessions)
• Foam rolling: IT band, thoracic spine, lats, calves (2 min each area)
• CARs (Controlled Articular Rotations): Every major joint — neck, shoulders, hips, ankles, wrists (5 rotations each direction)
• Deep squat hold: accumulate 5 minutes total (use support if needed; this restores hip mobility)
• Hanging from pull-up bar: accumulate 3–5 minutes total (decompresses spine, improves grip)

Daily Non-Negotiable Movement (every day including rest days)

• Morning: 10-minute walk outside within 30 minutes of waking (circadian entrainment)
• Post-meal walks: 10–15 minutes after each meal (dramatically improves glucose disposal — CGM will confirm)
• Movement snacks: Every 60 minutes of sitting, perform 2 minutes of: air squats, push-ups, or simply standing/walking
• Daily step target: 8,000–12,000 steps (track via wearable)

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1.4 SLEEP OPTIMIZATION — MONTH 1

Target: 7.5–8.5 hours of sleep opportunity (in bed), 7–8 hours actual sleep

Sleep Environment Engineering

Temperature:

• Bedroom temperature: 65–67°F (18.3–19.4°C)
• Consider: ChiliPad/Eight Sleep Pod (mattress cooling system) — set to 67°F at sleep onset, 65°F during deep sleep, warm to 68°F 30 min before wake
• Wear socks to bed if extremities are cold (vasodilation aids sleep onset)

Light:

• Complete blackout: blackout curtains + cover all LED lights with black electrical tape
• Target: cannot see your hand in front of your face
• If not possible: use a quality sleep mask (Manta Sleep mask)

Sound:

• White/brown noise machine (LectroFan) or brown noise app
• Or: silicone ear plugs (Mack's) if noise-sensitive

Air Quality:

• HEPA air purifier running in bedroom (Coway Airmega or Molekule)
• Humidity: 40–50% (use humidifier/dehumidifier as needed)
• Open window slightly if air quality permits (CO2 reduction)

Evening Routine (Begin 2 hours before target sleep time)

T-minus 2 hours (e.g., 8:30 PM for 10:30 PM bedtime):

• Blue light blocking glasses on (TrueDark Twilight or Ra Optics)
• Dim all house lights to <50 lux (use smart bulbs: Philips Hue set to warm 1,800K)
• No screens ideally; if necessary, use Night Shift + f.lux at maximum warmth
• Last meal should be finished

T-minus 1.5 hours:

• Evening supplement stack (magnesium glycinate, apigenin, L-theanine, tart cherry)
• Warm shower or bath (the subsequent body cooling triggers sleep onset)
• If using sauna, finish sauna session by this time

T-minus 1 hour:

• Journaling: 5-minute brain dump (write tomorrow's top 3 priorities)
• Gratitude practice: write 3 specific things from today
• Light reading (physical book, not Kindle with backlight)
• Gentle stretching or legs-up-the-wall pose (5 min)

T-minus 15 minutes:

• Nasal breathing practice: 4-7-8 breathing x 4 cycles
• Body scan meditation (use Yoga Nidra recording: NSDR — Non-Sleep Deep Rest)
• Room should be completely dark

Morning Wake Protocol

• Consistent wake time: ±30 minutes, even on weekends (this is the single most important sleep habit)
• No snooze button. Ever.
• First 5 minutes: Get outside for sunlight exposure (even on cloudy days, outdoor light is 10,000+ lux vs. 500 lux indoors)
• Sunlight exposure target: 10 minutes direct morning sun (no sunglasses); 20–30 minutes on cloudy days
• Delay caffeine: Wait 90–120 minutes after waking before first coffee (allows adenosine clearance and cortisol peak to pass)

Sleep Tracking Metrics (via Oura Ring or WHOOP)

Metric	Target	Notes
Total Sleep	7–8 hours	Actual sleep, not time in bed
Sleep Latency	<20 minutes	Time to fall asleep
REM Sleep	1.5–2 hours (20–25%)	Critical for memory consolidation
Deep Sleep (SWS)	1–1.5 hours (15–20%)	Growth hormone release, physical recovery
Sleep Efficiency	>85%	Time asleep / time in bed
HRV during sleep	Trending upward over weeks	Sign of recovery and parasympathetic tone
Resting HR during sleep	Trending downward	Cardiovascular efficiency
Wake episodes	<2 per night

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1.5 STRESS RESILIENCE & COGNITIVE ENHANCEMENT — MONTH 1

Daily Meditation Practice

Week 1–2: 10 minutes/day Week 3–4: 15 minutes/day

Protocol: Vipassana-style body scan or focused attention meditation

• App recommendation: Waking Up (Sam Harris) — foundational course
• Timing: Morning, after sunlight exposure, before first meal
• Position: Seated, spine erect, eyes closed or soft gaze downward

Breathwork Protocols

Daily Morning Breathwork (5–10 minutes, after meditation):

Wim Hof Method (Modified):

1. 30 deep breaths: full inhale through nose, passive exhale through mouth
2. After 30th exhale: hold breath (empty lungs) as long as comfortable
3. Recovery breath: deep inhale, hold 15 seconds
4. Repeat for 3 rounds
5. Finish with box breathing: 4-4-4-4 x 5 cycles

Benefits: Increases norepinephrine, reduces inflammation (proven in RCTs), improves stress tolerance, alkalizes blood temporarily, enhances focus.

Caution: Perform seated or lying down. Never in water or while driving.

HRV Training — Month 1: Baseline & Resonance Frequency

Equipment: Elite HRV app + compatible chest strap (Polar H10) or use Oura Ring passive HRV

Protocol:

1. Week 1–2: Morning HRV readings only (2-minute seated reading upon waking, before standing)

   • Track daily; establish 7-day rolling average
   • Note: HRV is highly individual; compare only to your own baseline

2. Week 3–4: Begin resonance frequency breathing

   • Use Elite HRV or Breathe+ app
   • Breathe at ~5.5–6 breaths per minute (typically ~5 sec inhale, ~5 sec exhale)
   • Practice for 10 minutes, 1x daily (can combine with morning meditation)
   • Goal: Find your personal resonance frequency (the breathing rate that maximizes HRV amplitude)

HRV Targets:

• Not about absolute number (varies by age, genetics)
• Goal: upward trend over 12 weeks
• Use HRV to guide training intensity: if HRV drops >15% below baseline, reduce training intensity that day

Cold Exposure — Month 1: Adaptation Phase

Protocol: Gradual Cold Water Immersion

Week 1: End showers with 30 seconds of cold water (as cold as tap allows) Week 2: 60 seconds cold at end of shower Week 3: 90 seconds cold; begin dedicated cold plunge if available (50–59°F / 10–15°C) Week 4: 2–3 minutes cold plunge at 50–59°F, 3x/week minimum

Timing: Morning preferred (dopamine increase lasts 3–5 hours) Key rules:

• Do NOT use cold within 4 hours after strength training (blunts hypertrophy signaling)
• Cold after Zone 2 cardio is fine
• Allow body to rewarm naturally (no hot shower after) — this extends the metabolic/dopamine benefits
• Focus on controlled nasal breathing during exposure

Benefits: 2.5x dopamine increase (lasting hours), norepinephrine surge, brown fat activation, improved insulin sensitivity, mental resilience, reduced inflammation.

Heat Exposure — Month 1

Sauna Protocol:

• Frequency: 3–4x/week
• Type: Finnish sauna preferred (dry, 170–212°F / 77–100°C); infrared sauna (130–150°F) acceptable
• Duration: 20–30 minutes per session (can split into 2–3 rounds of 10–15 min with 2-min cool breaks)
• Timing: Evening preferred (2–3 hours before bed; the subsequent body cooling aids sleep)

Hydration during sauna: 16 oz water with electrolytes before and after

Benefits: Heat shock protein activation (cellular repair), growth hormone increase (up to 16x in some studies with repeated exposure), cardiovascular conditioning (equivalent to moderate cardio), reduced all-cause mortality by 40% with 4–7x/week use (Finnish study, JAMA Internal Medicine 2015).

Journaling & Cognitive Practices

Daily (5 minutes, evening):

• 3 gratitudes (specific, not generic)
• 1 thing learned today
• Tomorrow's #1 priority

Weekly (Sunday, 20 minutes):

• Week review: wins, challenges, adjustments
• Rate week 1–10 on: energy, mood, sleep, training performance, cognitive clarity

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1.6 TRACKING & WEARABLES — MONTH 1

Wearable Stack

Device	Purpose	Key Metrics	When to Wear
Oura Ring (Gen 3)	Sleep, HRV, readiness, temperature	Sleep stages, HRV, resting HR, body temp trends	24/7 (especially sleep)
WHOOP 4.0 (alternative to Oura)	Strain, recovery, sleep	HRV, respiratory rate, strain score	24/7
Apple Watch Ultra / Garmin Fenix 7	Activity tracking, workout HR zones	Steps, active calories, HR zones during exercise, VO2max estimate	During waking hours + workouts
CGM (Continuous Glucose Monitor)	Glucose response to foods	Fasting glucose, post-meal spikes, glucose variability	Weeks 1–2 of each month (cycling)
Polar H10 Chest Strap	Accurate HR during workouts + HRV training	Real-time HR, HRV for biofeedback	During workouts and HRV sessions

CGM Protocol (Weeks 1–2)

Wear Abbott Libre 3 or Dexcom G7 continuously for 14 days

Goals:

• Fasting glucose: 72–85 mg/dL
• Post-meal peak: <120 mg/dL (ideally <110)
• Return to baseline: within 2 hours of eating
• Glucose variability (SD): <15 mg/dL
• Time in range (72–110): >90%
• No glucose spikes >140 mg/dL

Experiments to run during CGM wear:

1. Test your standard meals — identify which spike you most
2. Test the effect of a 10-min post-meal walk vs. no walk
3. Test meal order: vegetables first, then protein, then carbs last (Jessie Inchauspé protocol)
4. Test impact of vinegar (1 tbsp ACV in water before meals)
5. Test impact of sleep quality on next-day fasting glucose
6. Test individual foods: oatmeal, rice, sweet potato, berries, etc.

Document all findings in a spreadsheet or use Levels Health app for analysis

Daily Tracking Dashboard (use Notion, Google Sheets, or dedicated app)

Morning Metrics (within 15 min of waking):

• HRV (Oura/WHOOP readiness score)
• Resting heart rate
• Body weight (same scale, after bathroom, before water)
• Subjective scores (1–10): energy, mood, sleep quality, motivation
• Fasting glucose (from CGM or glucometer)

Evening Metrics (before bed):

• Total steps
• Training completed (Y/N, type, RPE)
• Nutrition adherence (1–10)
• Supplements taken (checklist)
• Stressors/notable events
• Gratitude entries

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MONTH 2: OPTIMIZATION & INTENSIFICATION (Weeks 5–8)

Theme: "Push the Envelope"

Primary Goals: Introduce advanced supplementation, intensify training, implement deeper fasting, add nootropic stack, begin neurofeedback concepts

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2.1 SUPPLEMENT STACK — MONTH 2: "Performance Stack"

Additions & Modifications to Month 1 Stack

Keep all Month 1 supplements, with these changes:

Change	Details
NMN → increase to 750 mg	Sublingual; split 500 AM + 250 midday
Add Fisetin	500 mg on days 1–3 of the month only (senolytic pulse)
Add Spermidine	10 mg/day with breakfast (autophagy enhancer)
Add Urolithin A (Mitopure)	500 mg/day (mitophagy — mitochondrial recycling; Timeline brand)
Add Tongkat Ali	400 mg/day (standardized 2% eurycomanone; Nootropics Depot) — 5 days on, 2 days off
Add Cistanche	200 mg/day (pairs with Tongkat Ali for hormonal optimization)

NEW: Nootropic/Cognitive Stack (Month 2)

Morning Cognitive Stack (taken with first meal):

Supplement	Dosage	Purpose
Lion's Mane (dual extract)	1,000 mg	NGF stimulation, neuroplasticity
Bacopa Monnieri (standardized 50% bacosides)	300 mg	Memory consolidation, anxiolytic
Phosphatidylserine	200 mg	Cortisol modulation, cognitive support
CDP-Choline (Citicoline)	250 mg	Acetylcholine precursor, focus

As-Needed Focus Stack (for demanding cognitive work):

• Caffeine 100 mg + L-Theanine 200 mg (capsule or matcha tea)
• Take no later than 1:00 PM
• Max 2x/week to prevent tolerance

Senolytic Pulse Protocol (First 3 days of Month 2 only)

Day	Supplement	Dosage	Notes
Days 1–3	Fisetin	500 mg (20 mg/kg for more aggressive protocol)	Take with fat (EVOO); Swanson or Doctor's Best
Days 1–3	Quercetin Phytosome	1,000 mg (doubled from maintenance)	Combined with fisetin for synergistic senolytic effect

Purpose: Clear senescent "zombie" cells that accumulate with age, secreting inflammatory SASP factors. Pulsed dosing mimics the protocol used in Mayo Clinic research. Do not take senolytics continuously.

Cycling Schedule — Month 2

Supplement	Schedule
NMN	Daily (no cycling needed)
Resveratrol	Daily
Tongkat Ali	5 days on / 2 days off
Lion's Mane	Daily for Month 2, then 1 week off at start of Month 3
Bacopa	Daily (requires 8–12 weeks for full effect; do not cycle)
Fisetin + high-dose Quercetin	Days 1–3 only, then return to maintenance quercetin dose
All others	Daily

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2.2 DIETARY PROTOCOL — MONTH 2: "Metabolic Flexibility"

Protocol: Carb Cycling + Extended Fasting Integration

Eating Window: Tighten to 18:6 on rest days, maintain 16:8 on training days

Carb Cycling Framework

Day Type	Carbs	When
Heavy Training Days (Mon, Wed, Fri)	150–200g	Concentrated around training (75% of carbs within 3 hours of workout)
Zone 2 Cardio Days (Tue, Sat)	75–100g	Fasted cardio in AM; carbs at lunch and dinner
Rest Days (Thu, Sun)	<50g (ketogenic)	Promotes metabolic flexibility and ketone production

New Additions to Diet

Polyphenol-Rich Foods (increase):

• Dark chocolate: 1 oz daily (>85% cacao; Hu Kitchen or Lindt 90%)
• Green tea / matcha: 2–3 cups daily (EGCG for AMPK activation)
• Pomegranate juice: 4 oz daily or pomegranate seeds (urolithin A precursor)
• Turmeric + black pepper: add to cooking daily (curcumin absorption)

Organ Meats (introduce if not already consuming):

• Beef liver: 3–4 oz, 1–2x/week (nature's multivitamin: retinol, B12, folate, copper, CoQ10)
• If taste is an issue: freeze liver, grate frozen into ground beef, or use Ancestral Supplements desiccated liver capsules (6/day)

Glycine Loading:

• Add 5g glycine powder to evening tea or bone broth
• Benefits: collagen synthesis, sleep quality (acts on NMDA receptors), glutathione precursor

Month 2 Extended Fast: 48-Hour Water Fast (Week 7)

Preparation (Day before):

• Eat a normal dinner, slightly lower carb
• Hydrate well
• Take electrolytes

During the 48-hour fast:

• Water: 2–3 liters/day with electrolytes (sodium, potassium, magnesium)
• Black coffee and plain tea allowed
• Electrolyte recipe: per liter of water add ½ tsp salt, ¼ tsp potassium chloride (NoSalt), 1 tsp magnesium citrate powder
• Light walking OK; no intense exercise
• Monitor glucose with CGM (expect 60–75 mg/dL range)
• Monitor ketones with blood ketone meter (target: 1.5–3.0 mmol/L by hour 36+)
• Continue NMN and basic supplements; pause fat-soluble supplements (need food for absorption)

Breaking the fast (critical):

• Hour 0: Bone broth (1 cup, warm)
• Hour 1: Small meal — 2 eggs, ½ avocado, sauerkraut
• Hour 3: Normal-sized meal, moderate protein and fat, moderate carbs
• Resume normal eating by next meal

Benefits of 48-hour fast: Deep autophagy, stem cell regeneration (Valter Longo research), insulin sensitivity reset, gut lining renewal, immune system reboot, BDNF increase.

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2.3 EXERCISE PROGRAMMING — MONTH 2: "Intensify"

Key Changes from Month 1

1. Add 1 dedicated VO2max session per week (replacing Saturday Zone 5 finisher with a standalone session)
2. Increase strength training volume (add 1 set to main lifts, introduce progressive overload tracking)
3. Add plyometric/power work
4. Introduce grip strength specific training (longevity marker)

Updated Weekly Structure

Day	Focus	Duration
Monday	Strength A — Upper Hypertrophy	60 min
Tuesday	Zone 2 Cardio	50–60 min
Wednesday	Strength B — Lower Hypertrophy	60 min
Thursday	VO2max Intervals + Core	35–40 min
Friday	Strength C — Full Body Strength/Power	60 min
Saturday	Zone 2 Cardio (long session)	60–75 min
Sunday	Active Recovery: Yoga/Mobility + Nature Walk	45–60 min

VO2max Interval Protocol (Thursday)

The "4x4 Norwegian Protocol" (proven to improve VO2max in research):

Warm-up: 10 minutes easy cardio (bike or rower)

Main set:

• 4 x 4 minutes at 90–95% max HR (Zone 5; should be VERY hard, unable to speak more than a few words)
• 3 minutes active recovery at 60–70% max HR between intervals
• Total work time: 16 minutes high intensity

Cool-down: 5 minutes easy + stretching

Modality: Rowing machine, assault bike, or uphill running (bike preferred for joint preservation)

Why VO2max matters: Moving from "low" to "above average" VO2max is associated with a 5x reduction in all-cause mortality. It is the single most powerful predictor of longevity (Peter Attia, Outlive).

Grip Strength Protocol (add to 2 training days/week)

Exercise	Sets x Duration/Reps	Notes
Dead hang from pull-up bar	3 x max hold	Target accumulating 3+ min total
Plate pinch hold	3 x 30 sec	Pinch two 10-lb plates together
Fat Gripz on pulling exercises	Use on 1 pulling exercise per session	Increases grip demand
Hand gripper (Captains of Crush)	3 x 8–10 reps each hand	Start with "Sport" or "Trainer" level

Progressive Overload Tracking

Use a training log (app: Strong, or Google Sheets) to track:

• Exercise, weight, sets, reps, RPE for every session
• Month 2 goal: increase weight on main lifts by 5–10% or add 1–2 reps at same weight
• Follow double progression: when you hit the top of rep range for all sets, increase weight by smallest increment

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2.4 ADVANCED STRESS RESILIENCE — MONTH 2

Meditation Upgrade

Duration: 20 minutes/day Add: 1x weekly "long sit" — 30–45 minutes on Sunday

New technique introduction: Open monitoring / choiceless awareness

• After 10 minutes of focused attention (breath), release focus
• Simply observe whatever arises: thoughts, sounds, sensations
• Non-judgmental awareness — this builds metacognition and emotional regulation

Neurofeedback Concepts — Month 2

Option A: Professional Neurofeedback (if accessible)

• Find a neurofeedback practitioner offering qEEG-guided training
• Protocols: SMR (sensorimotor rhythm) training for focus, alpha training for calm alertness
• Frequency: 1–2 sessions/week
• Cost: ~$100–200/session

Option B: At-Home Neurofeedback (more accessible)

• Device: Muse 2 headband (~$250)
• Protocol: Use Muse app for guided meditation with real-time EEG feedback
  • 10–15 minutes daily
  • Track "calm" percentage over time
  • Goal: increase calm percentage from baseline by 20%+ over Month 2–3

Option C: Focus@Will or Brain.fm

• Use neuroscience-based audio for work sessions
• Not true neurofeedback but leverages auditory entrainment for focus states

Advanced Cold Exposure — Month 2

Upgrade protocol:

• Temperature: 40–50°F (4–10°C) — colder than Month 1
• Duration: 3–5 minutes
• Frequency: 4–5x/week
• Introduce: deliberate calm during cold (controlled nasal breathing, resist urge to hyperventilate)
• New addition: Contrast therapy — alternate 3 min cold → 10 min sauna → 3 min cold → 10 min sauna (2–3 rounds)

Vagus Nerve Stimulation Techniques

Daily practices (choose 2–3):

1. Gargling: Gargle water vigorously for 30 seconds, 2x/day (stimulates vagus via pharyngeal muscles)
2. Cold face immersion: Submerge face in bowl of ice water for 15–30 seconds (triggers dive reflex, activates vagus)
3. Humming/chanting: 5 minutes of "Om" chanting or humming (vibration stimulates vagus)
4. Slow exhale breathing: 5.5 breaths/min, emphasizing long exhale (resonance frequency breathing)
5. Singing loudly: Activates vagus via vocal cord engagement

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MONTH 3: INTEGRATION & PEAK PERFORMANCE (Weeks 9–12)

Theme: "Sustain & Transcend"

Primary Goals: Consolidate gains, test peak performance, establish sustainable long-term protocols, retest biomarkers, optimize based on data

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3.1 SUPPLEMENT STACK — MONTH 3: "Integration Stack"

Modifications

Change	Details
NMN: maintain 750 mg	Reassess based on NAD+ test if retested
Add Rapamycin (if physician-supervised)	5–6 mg once weekly (sirolimus); requires prescription and medical oversight; this is the most evidence-backed longevity drug in animal models
Add Metformin OR Berberine (choose one)	Metformin: 500 mg with dinner (Rx required) OR Berberine: 500 mg 2x/day with meals
Cycle off Lion's Mane	Week 9 off, resume Week 10–12
Add Astaxanthin	12 mg/day
Add PQQ (Pyrroloquinoline quinone)	20 mg/day
Continue all other Month 2 supplements	Maintain cycling schedules

Important Note on Rapamycin

Rapamycin (sirolimus) is a prescription immunosuppressant being studied off-label for longevity. It inhibits mTOR, promoting autophagy and extending lifespan in every organism tested. This should ONLY be pursued under physician supervision (longevity-focused doctors like those at Attia's practice, AgelessRx, or similar). Weekly low-dose pulsing is the protocol used in human longevity contexts. If not comfortable with Rx interventions, skip this and rely on natural mTOR modulation via fasting and exercise.

Month 3 Senolytic Pulse #2 (Week 11, Days 1–3)

Repeat the fisetin + quercetin senolytic pulse from Month 2:

• Fisetin 500 mg + Quercetin 1,000 mg for 3 consecutive days
• Then return to maintenance quercetin (500 mg/day)

Complete Daily Supplement Schedule — Month 3

Upon Waking (6:30 AM, with water):

• Nothing (wait for morning routine)

With Meal 1 (11:00 AM):

• NMN 500 mg (sublingual 10 min before eating)
• Trans-Resveratrol 500 mg
• Vitamin D3 5,000 IU + K2 200 mcg
• Omega-3 2g EPA/1g DHA
• Creatine 5g
• B-Complex (methylated)
• Vitamin C 1,000 mg
• Lion's Mane 1,000 mg
• Bacopa 300 mg
• CDP-Choline 250 mg
• Spermidine 10 mg
• Urolithin A 500 mg
• Tongkat Ali 400 mg (weekdays only)
• Astaxanthin 12 mg

With Meal 2 (2:30 PM):

• NMN 250 mg (sublingual)
• Sulforaphane 15 mg
• CoQ10 200 mg (ubiquinol)
• PQQ 20 mg
• R-ALA 300 mg
• Quercetin 500 mg
• Phosphatidylserine 200 mg
• Cistanche 200 mg

Evening (6:30 PM with dinner or before bed):

• Berberine 500 mg (with dinner) OR Metformin 500 mg (if using)
• Magnesium Glycinate 400 mg
• Magnesium L-Threonate 1,000 mg
• Apigenin 50 mg
• L-Theanine 200 mg
• Glycine 5g (in tea or water)
• Tart Cherry Extract 500 mg

Weekly (if using):

• Rapamycin 5–6 mg: Sunday morning, fasted (physician-supervised)

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3.2 DIETARY PROTOCOL — MONTH 3: "Precision Nutrition"

Protocol: Personalized Carb Cycling + OMAD Days + Quarterly Extended Fast

Based on Month 1–2 CGM data, you should now know:

• Your personal glycemic responses to specific foods
• Optimal meal timing for glucose control
• Which carb sources you tolerate best
• Impact of exercise timing on glucose

Week 9–10: Refined Carb Cycling

Same framework as Month 2, but now personalized:

• High carb training days: Emphasize your best-tolerated carb sources (per CGM data)
• Keto rest days: Target blood ketones 0.5–1.5 mmol/L (verify with blood ketone meter — Keto-Mojo)
• Add 1 OMAD (One Meal A Day) day per week — typically Sunday
  • Single large meal: 1,500–2,000 kcal
  • High protein (60–80g), moderate fat, moderate carb
  • 23-hour fast provides deep autophagy benefits

Week 11: 72-Hour Water Fast (Quarterly Extended Fast)

This is the capstone fasting event of the 3-month protocol.

Days 1–3 of Week 11:

Preparation (day before):

• Light dinner, lower carb
• Prepare electrolyte water
• Clear schedule of demanding social food events

During the fast:

• Water with electrolytes (same recipe as Month 2)
• Black coffee (1–2 cups max, before noon)
• Herbal tea
• ½ tsp salt under tongue if feeling lightheaded
• Light walking only (20–30 min/day)
• Monitor: glucose, ketones, blood pressure, weight, subjective well-being
• Expected ketone levels by hour 48–72: 3.0–5.0 mmol/L
• Expected glucose: 55–70 mg/dL (this is safe in a fasting context for healthy individuals)
• Continue: basic supplements (NMN, electrolytes, magnesium)
• Pause: fat-soluble supplements, berberine/metformin, protein-based supplements

Breaking the fast (Day 4 — critical for safety and gut comfort):

• Hour 0: Warm bone broth (1 cup)
• Hour 2: Small meal — 2 soft-boiled eggs, ½ avocado, small portion sauerkraut
• Hour 5: Moderate meal — 4 oz salmon, steamed vegetables, small sweet potato
• Hour 8+: Resume normal eating
• Expect vivid mental clarity and enhanced insulin sensitivity for days after

Contraindications: Do NOT attempt 72-hour fasts if you have a history of eating disorders, are underweight (BMI <18.5), are pregnant/nursing, have type 1 diabetes, or are on medications that require food. Consult physician.

Week 12: Return to Optimized Baseline Eating

• Resume your refined 16:8 protocol with carb cycling
• This becomes your sustainable long-term dietary framework
• 1 OMAD day per week (optional, based on preference)
• Quarterly 48–72 hour fasts going forward

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3.3 EXERCISE PROGRAMMING — MONTH 3: "Peak & Test"

Key Changes

1. Deload Week 9 (reduce volume by 40%, maintain intensity) — allows supercompensation
2. Peak performance testing Week 10–11
3. Introduction of rucking as Zone 2 modality
4. Add stability and balance training (fall prevention = longevity)

Updated Weekly Structure

Day	Focus	Duration
Monday	Strength A — Upper (Peak Phase)	60 min
Tuesday	Zone 2: Rucking (30 lb pack, outdoor)	60 min
Wednesday	Strength B — Lower (Peak Phase)	60 min
Thursday	VO2max Intervals (Norwegian 4x4)	35 min
Friday	Strength C — Full Body + Stability	60 min
Saturday	Zone 2: Long Ruck or Bike	75–90 min
Sunday	Active Recovery: Yoga + Balance Training	45 min

Week 9: Deload Protocol

• Same exercises as Month 2
• Reduce all working weights by 40%
• Reduce sets by 1 (e.g., 4 sets → 3 sets)
• Focus on movement quality, mind-muscle connection, tempo
• Maintain Zone 2 cardio volume
• Skip VO2max session (replace with easy Zone 2)

Weeks 10–11: Performance Testing

Test the following and compare to Phase 0 baseline:

Test	Protocol	Target Improvement
1RM or estimated 1RM (Bench, Squat, Deadlift)	Work up to heavy single or use RPE-based estimation	5–15% increase
VO2max (or proxy)	12-min Cooper Run Test or lab VO2max retest	5–10% improvement
Grip strength	3 attempts each hand, dynamometer	5–10% improvement
Resting heart rate	7-day average from wearable	3–5 bpm decrease
HRV	7-day average from wearable	10–20% increase
Body composition	DEXA scan retest	Decrease body fat 1–3%, maintain/gain lean mass
Dead hang	Max time	Target: 90+ seconds
Deep squat hold	Max time	Target: 5+ minutes

Balance & Stability Training (added to Sunday sessions)

Exercise	Duration/Reps	Progression
Single-leg stand (eyes open)	60 sec each leg	Progress to eyes closed
Single-leg stand (eyes closed)	30 sec each leg	Progress to unstable surface (BOSU)
Tandem walk (heel-to-toe)	20 steps forward + backward	Add head turns
Single-leg Romanian deadlift (unloaded)	10 reps each leg	Add light dumbbell
Turkish get-up	3 per side, light KB	Focus on control and stability
Bosu ball squats	3 x 10	Progress to single leg

Why balance training matters for longevity: Inability to stand on one leg for 10 seconds in adults over 50 is associated with an 84% increased risk of death (British Journal of Sports Medicine, 2022). Falls are a leading cause of death in older adults.

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3.4 ADVANCED STRESS RESILIENCE — MONTH 3

Meditation: 20–25 minutes daily

Add: Loving-kindness (Metta) meditation 1x/week

• Generates positive emotional states
• Research shows it increases vagal tone and social connection
• Protocol: Systematically send wishes of well-being to self → loved ones → neutral people → difficult people → all beings

Advanced Breathwork — Month 3

Add: Cyclic Hyperventilation + Retention (weekly)

• 3 rounds of 30 breaths + max retention (Wim Hof style)
• Followed by 5 minutes of box breathing (4-4-4-4)
• Then 5 minutes of resonance frequency breathing
• Total session: ~20 minutes
• Track retention times (expect improvement from Month 1)

Add: CO2 Tolerance Training

• Exhale, then time how long you can hold before the urge to breathe
• Baseline: most people 20–30 seconds
• Target: 60+ seconds (indicates high CO2 tolerance, better stress resilience)
• Practice: daily, 3 attempts, track longest hold

Psychedelic-Assisted Neuroplasticity (Legal Alternatives)

Note: This section covers legal approaches only.

Option A: Psilocybin Microdosing (where legal — e.g., Oregon, Netherlands, Jamaica)

• Protocol: Fadiman Protocol — dose every 3rd day
• Dose: 50–100 mg dried psilocybin mushrooms (sub-perceptual)
• Duration: 4–6 weeks
• Benefits: enhanced neuroplasticity, creativity, mood, default mode network modulation
• Track: mood, creativity, social connection, cognitive performance

Option B: Legal Neuroplasticity Enhancement

• Lion's Mane (already in stack) — promotes NGF
• BDNF enhancement: High-intensity exercise, sauna, cold exposure (all in protocol)
• Novel experiences: Dedicate 1 day/week to something completely new (new skill, new route, new food, new social group)
• Non-dominant hand practice: Brush teeth, eat, write with non-dominant hand for 5 min/day

Nature Exposure Protocol

Minimum: 120 minutes/week in natural environments (forest, park, waterfront)

• Research (White et al., 2019, Scientific Reports): 120 min/week in nature associated with significantly better health and well-being
• Combine with Zone 2 cardio (rucking in nature = double benefit)
• Practice "forest bathing" (shinrin-yoku): slow, mindful walking in forest, engaging all senses
• Leave phone on airplane mode during nature sessions

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3.5 ADVANCED TRACKING & RETESTING — MONTH 3

Week 10: Retest CGM (14-day wear)

• Compare glucose metrics to Month 1 baseline
• Expected improvements: lower fasting glucose, reduced post-meal spikes, tighter variability, higher time-in-range

Week 11–12: Comprehensive Lab Retest

Retest the full blood panel from Phase 0:

Expected improvements after 3 months:

Marker	Expected Direction	Notes
HbA1c	↓ 0.1–0.3%	Reflects 3-month glucose average
Fasting insulin	↓	Improved insulin sensitivity
hs-CRP	↓	Reduced systemic inflammation
Homocysteine	↓	Improved methylation (B vitamins)
Triglycerides	↓	Reduced carb intake, improved fat metabolism
HDL-C	↑	Exercise + healthy fats
ApoB	↓ or stable	Monitor; if elevated, discuss with physician
Vitamin D	↑ to 60–80 ng/mL	Supplementation effect
Omega-3 Index	↑ to >8%	Supplementation effect
Testosterone (free)	↑	Tongkat Ali, sleep, exercise, stress reduction
Cortisol (AM)	Optimal range	Stress resilience practices
RBC Magnesium	↑	Supplementation effect
NAD+ (intracellular)	↑	NMN supplementation
Biological age (TruAge)	↓ 1–3 years	Epigenetic clock; most exciting metric

Week 12: Performance Retesting

Retest all functional assessments from Phase 0 and Week 10–11 testing:

• VO2max
• DEXA scan
• Grip strength
• HRV trends
• Cognitive assessment (same platform as baseline)
• Biological age test

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COMPREHENSIVE WEEKLY SCHEDULE TEMPLATE

Sample Week — Month 2 (Peak Protocol)

MONDAY

Time	Activity	Details
6:00 AM	Wake	Consistent time
6:05 AM	Morning light	10 min outdoor walk, no sunglasses
6:20 AM	HRV reading	2-min seated reading (Elite HRV + Polar H10)
6:25 AM	Breathwork	Wim Hof 3 rounds (15 min)
6:45 AM	Meditation	20 min focused attention (Waking Up app)
7:05 AM	Cold exposure	3 min cold plunge (45°F)
7:15 AM	Allow natural rewarming	Light movement, journaling
7:30 AM	Morning hydration	16 oz water + electrolytes + morning supplements (NMN sublingual)
8:00–10:30 AM	Deep work block	Fasted; black coffee at 8 AM if desired; phone on DND
11:00 AM	Meal 1 (break-fast)	Training day meal + AM supplement stack
11:30 AM–2:00 PM	Work / meetings	Post-meal 10-min walk at 11:45
2:30 PM	Meal 2 (pre-training)	Moderate carbs + protein + midday supplements
3:30 PM	Gym: Strength A (Upper)	60 min; log all lifts in Strong app
4:30 PM	Post-workout shake	30g whey + berries + creatine
5:00 PM	Sauna	20 min at 185°F; hydrate with electrolytes
5:30 PM	Post-meal walk / errands	15 min
6:30 PM	Meal 3 (dinner)	Anti-inflammatory dinner + evening supplements
7:00 PM	Feeding window closes
7:30 PM	Evening routine begins	Blue light blockers on; dim lights
8:00 PM	Quality time / reading / light hobby	No screens ideally
9:00 PM	Evening supplements	Mg glycinate, apigenin, L-theanine, glycine
9:15 PM	Warm shower/bath
9:30 PM	Journaling	Brain dump + gratitude
9:45 PM	NSDR / Yoga Nidra	10 min recording
10:00 PM	Lights out	Target: asleep by 10:20 PM

TUESDAY (Zone 2 Day)

Time	Activity
6:00 AM	Wake, morning light, HRV
6:30 AM	Fasted Zone 2 cardio: 50 min incline treadmill walk or outdoor ruck
7:20 AM	Cold shower (2 min cold finish)
7:30 AM	Meditation (20 min)
8:00 AM	Hydration + morning supplements
11:00 AM	Meal 1
Evening	Sauna (20 min) if not done post-cardio
10:00 PM	Lights out

THURSDAY (VO2max + Recovery Day)

Time	Activity
6:00 AM	Wake, morning light, HRV
6:30 AM	Meditation (20 min) + breathwork (10 min)
7:00 AM	Morning supplements
11:00 AM	Meal 1
2:30 PM	Meal 2
3:30 PM	VO2max intervals (Norwegian 4x4): 35 min total
4:15 PM	Mobility work: foam rolling + CARs (20 min)
5:00 PM	Contrast therapy: cold plunge 3 min → sauna 10 min → cold 3 min → sauna 10 min
6:30 PM	Meal 3 + evening supplements
10:00 PM	Lights out

SUNDAY (Recovery + Reflection)

Time	Activity
6:30 AM	Wake (allow slight sleep-in but within 30 min of usual)
6:35 AM	Morning light walk (20 min, nature if possible)
7:00 AM	Extended meditation (30–45 min; Metta or long Vipassana sit)
7:45 AM	Breathwork (resonance frequency, 10 min)
8:00 AM	Journaling: weekly review (20 min)
11:00 AM	Meal 1 (OMAD day in Month 3 — this becomes the single large meal)
12:00 PM	Nature walk / forest bathing (60–90 min)
2:00 PM	Yoga / mobility / balance training (45 min)
3:00 PM	Leisure: reading, socializing, hobby
5:00 PM	Sauna (20 min)
5:30 PM	Cold plunge (3 min)
6:30 PM	Light meal (if not OMAD) + evening supplements
9:00 PM	Prepare for week: meal prep, supplement organizer, review schedule
10:00 PM	Lights out

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ENVIRONMENTAL & LIFESTYLE OPTIMIZATION

Air Quality

• HEPA air purifier in bedroom and office (Coway Airmega 400)
• Monitor AQI; avoid outdoor exercise when AQI >100
• Houseplants: pothos, snake plant, spider plant (mild air filtering + biophilic benefit)
• Avoid: scented candles, air fresheners, conventional cleaning products (VOCs)
• Use: Branch Basics or Force of Nature for cleaning

Water Quality

• Whole-house water filter or countertop reverse osmosis (AquaTru)
• Remineralize RO water: add trace mineral drops (ConcenTrace)
• Avoid: plastic water bottles (BPA/BPS, microplastics)
• Use: glass or stainless steel bottles (Hydroflask, Klean Kanteen)
• Daily water intake: 0.5 oz per pound of body weight + additional for exercise and sauna

Light Environment

• Morning: maximize bright light (outdoor sunlight or 10,000 lux light therapy box if dark climate — Carex Day-Light)
• Daytime: work near windows; take outdoor breaks
• Evening: smart bulbs set to 1,800K warm after sunset (Philips Hue)
• Night: complete darkness for sleep
• Screen filters: f.lux on computer, Night Shift on phone, or physical blue light filter

EMF Reduction (precautionary)

• Phone on airplane mode during sleep
• WiFi router on timer (off during sleep hours) or use ethernet for desktop
• Phone not in pocket against body (use bag or desk)
• No phone/laptop on lap near reproductive organs

Social Connection (often overlooked longevity factor)

• Research: Social isolation increases mortality risk by 26% (Holt-Lunstad meta-analysis)
• Schedule: minimum 2 meaningful social interactions per week
• Prioritize in-person over digital
• Consider: joining a fitness community, meditation group, or mastermind
• Maintain/cultivate 3–5 deep relationships

Toxin Reduction

• Food: Organic for Dirty Dozen produce; pasture-raised animal products
• Personal care: EWG Skin Deep database — switch to clean products (score 1–2)
• Cookware: Cast iron, stainless steel, ceramic — eliminate non-stick/Teflon
• Storage: Glass containers (Pyrex) — eliminate plastic food storage
• Dental: Biological dentist; consider mercury amalgam removal if present

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LONG-TERM SUSTAINABILITY FRAMEWORK

After the 3-Month Protocol

What to Continue Indefinitely

• Zone 2 cardio: 150+ min/week (non-negotiable)
• Strength training: 3x/week (non-negotiable)
• Sleep optimization: 7–8 hours, consistent schedule
• Morning sunlight: 10+ minutes daily
• Meditation: 10–20 min daily
• Cold exposure: 3x/week (11 min total/week minimum per Huberman)
• Sauna: 3–4x/week
• Time-restricted eating: 16:8 minimum
• Core supplement stack: NMN, D3/K2, Omega-3, Magnesium, Creatine
• Quarterly 48–72 hour fasts or FMD
• Bi-annual comprehensive blood work
• Annual: DEXA, VO2max test, biological age test

What to Cycle

• Nootropic stack: 8 weeks on, 2 weeks off
• Tongkat Ali: 5 on/2 off ongoing
• Senolytic pulses: every 2–3 months (3-day fisetin + quercetin)
• Rapamycin (if using): ongoing weekly under physician supervision with periodic labs

Quarterly Reviews

• Full blood panel
• DEXA scan
• CGM wear (2 weeks)
• Adjust supplement dosages based on labs
• Adjust training based on progress and goals
• Review and update dietary protocol based on CGM data and body composition

Annual Advanced Testing

• Biological age test (TruAge or GlycanAge)
• Full-body MRI (Prenuvo — screen for early cancer and structural issues)
• Coronary artery calcium (CAC) score (after age 40)
• Comprehensive cognitive assessment
• CPET (cardiopulmonary exercise test) for true VO2max

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COST ESTIMATE (Monthly)

Category	Estimated Monthly Cost (USD)
Supplements (full stack)	$350–500
Food (high-quality, organic)	$600–900
Gym membership	$50–100
Wearables (amortized)	$30–50
CGM sensors (when used)	$75–150
Sauna access (gym or home)	$0–100
Lab testing (amortized quarterly)	$100–200
Cold plunge (amortized or gym)	$0–50
Apps/subscriptions	$20–40
Total	$1,225–2,090/month

Note: Costs can be significantly reduced by prioritizing the highest-impact interventions: sleep, Zone 2 cardio, strength training, and basic supplements (D3, Omega-3, Magnesium, Creatine) — which cost under $200/month total.

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QUICK-REFERENCE: TOP 10 HIGHEST-IMPACT INTERVENTIONS

If you can only do 10 things from this entire protocol, do these:

1. Sleep 7–8 hours with consistent wake time and dark/cool room
2. Zone 2 cardio 150+ minutes per week
3. Strength train 3x per week with progressive overload
4. Morning sunlight 10+ minutes within 30 min of waking
5. Time-restricted eating (16:8) with whole foods, high protein
6. Supplement: Vitamin D3 (to 60+ ng/mL), Omega-3 (to Index >8%), Magnesium, Creatine
7. Daily meditation/breathwork 10–20 minutes
8. Cold exposure 11+ minutes per week total
9. Walk after meals 10 minutes
10. Maintain strong social connections

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DISCLAIMER

This protocol is for educational purposes and designed for generally healthy adults interested in optimization. It is not medical advice. Before implementing any supplement, fasting, exercise, or pharmaceutical protocol (especially rapamycin, metformin, or any prescription medication), consult with a qualified healthcare provider. Individual responses vary based on genetics, health status, medications, and other factors. Start conservatively, titrate up, and listen to your body. Blood work should be interpreted by a qualified professional. The author assumes no liability for individual outcomes.

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Version: 3.0 Last Updated: Based on evidence available through mid-2025 Key Influences: Peter Attia (Outlive), David Sinclair (Lifespan), Andrew Huberman (Huberman Lab), Bryan Johnson (Blueprint), Valter Longo (fasting research), Rhonda Patrick (FoundMyFitness), Andy Galpin (exercise science)

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This document represents a synthesis of current best evidence in longevity science. The field evolves rapidly. Stay curious, stay skeptical, measure everything, and adjust based on YOUR data.