The Comprehensive 3-Month Longevity Optimization Protocol

Overview & Foundation

This protocol integrates cutting-edge biohacking strategies with evidence-based longevity research. Each com ponent is designed to work synergistically for maximum healthspan and performance optimization.

Core Pillars:

• Metabolic optimization through targeted nutrition
• Hormetic stress application
• Recovery and regeneration maximization
• Cognitive enhancement
• Biomarker tracking and adjustment

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Phase 1: Foundation Building (Weeks 1-4)

Dietary Protocol

Week 1-2: Metabolic Flexibility Training

• Daily Structure:
  • 16:8 Intermittent Fasting (eating window: 12pm-8pm)
  • Break fast with MCT oil (15ml) + electrolytes
  • Meal 1 (12pm): High protein, moderate fat, low carb
  • Meal 2 (6pm): Complete amino acid profile, healthy fats

Week 3-4: Modified Ketogenic Approach

• Macronutrient Ratios:
  • 70% fat, 25% protein, 5% carbs
  • Total calories: BMR × 1.2-1.4 (depending on activity)
• Carb Cycling: 20g net carbs 5 days, 50-100g on training days
• Key Foods:
  • Grass-fed beef, wild salmon, pasture-raised eggs
  • Avocados, MCT oil, extra virgin olive oil
  • Leafy greens, cruciferous vegetables
  • Macadamia nuts, Brazil nuts (2-3 daily for selenium)

Supplement Stack - Phase 1

Morning Stack (7am):

• NAD+ Precursor (Nicotinamide Riboside): 300mg
• Resveratrol: 500mg (with fat for absorption)
• Curcumin (with piperine): 1000mg
• Vitamin D3: 4000 IU + K2 (MK-7): 200mcg
• Magnesium Glycinate: 400mg
• Omega-3 (EPA/DHA): 2g

Pre-Workout (30min before exercise):

• Creatine Monohydrate: 5g
• Beta-Alanine: 3g
• L-Citrulline: 6g
• Rhodiola Rosea: 300mg

Evening Stack (8pm):

• Magnesium Threonate: 2g
• Melatonin: 0.5mg (micro-dose)
• Glycine: 3g
• Ashwagandha (KSM-66): 600mg

Weekly Additions:

• Spermidine: 1mg (3x/week)
• Rapamycin: 5mg (once weekly - consult physician)

Exercise Protocol - Phase 1

Monday - Strength Training (Lower Body Focus):

• Warm-up: 10min dynamic movement
• Squats: 4×6-8 (70-80% 1RM)
• Romanian Deadlifts: 3×8-10
• Bulgarian Split Squats: 3×10 each leg
• Calf Raises: 3×15
• Core Circuit: 10min
• Cool-down: 10min stretching

Tuesday - HIIT Cardio:

• 5min warm-up
• 8×30sec all-out / 90sec recovery
• 5min cool-down
• Total: 25min

Wednesday - Active Recovery:

• 30min zone 2 cardio (nasal breathing only)
• Yoga or mobility work: 20min
• Sauna: 15-20min at 80-90°C

Thursday - Strength Training (Upper Body):

• Pull-ups/Chin-ups: 4×6-8
• Overhead Press: 4×6-8
• Rows: 3×8-10
• Dips: 3×10-12
• Bicep/Tricep superset: 3×12

Friday - Zone 2 Cardio:

• 45-60min at 60-70% max HR
• Monitor via HRV device

Saturday - Functional Movement:

• Compound movements
• Farmer's walks, Turkish get-ups
• Balance and coordination drills
• 45min total

Sunday - Complete Rest or Gentle Movement

Stress Resilience & Recovery - Phase 1

Daily HRV Training:

• Morning HRV measurement (HeartMath Pro, WHOOP, or Oura)
• 10min coherent breathing (5sec in, 5sec out)
• Adjust training intensity based on HRV trends

Weekly Protocols:

• Sauna: 3×/week, 15-20min at 80-90°C
• Cold exposure: 3×/week, 2-3min cold shower (15°C or below)
• Meditation: Daily 10-20min (Headspace or Waking Up app)

Sleep Optimization:

• Consistent sleep/wake times (±30min)
• Blue light blocking glasses after sunset
• Room temperature: 18-19°C
• Complete darkness (blackout curtains, eye mask)
• Sleep tracking via Oura Ring or WHOOP

Tracking & Biomarkers - Phase 1

Daily Tracking:

• HRV (morning)
• Sleep quality and duration
• Ketone levels (blood or breath)
• Glucose (fasting and post-meal)
• Energy levels (1-10 scale)
• Cognitive performance (simple tests)

Week 4 Lab Panel:

• Complete Blood Count (CBC)
• Comprehensive Metabolic Panel (CMP)
• Lipid panel (including particle size)
• HbA1c and fasting insulin
• Inflammatory markers (CRP, IL-6)
• Hormone panel (testosterone, cortisol, thyroid)
• Vitamin D, B12, folate
• Omega-3 index

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Phase 2: Optimization (Weeks 5-8)

Advanced Dietary Protocol

Cyclic Ketogenic Diet with Targeted Carb Timing:

• 5 days ketogenic: <20g net carbs
• 1 day moderate carb: 100-150g (training day)
• 1 day higher carb: 200-250g (refeed)

Intermittent Fasting Progression:

• Week 5-6: 18:6 protocol
• Week 7-8: Two 24-hour fasts per week

Meal Timing Optimization:

• Post-workout: Protein + targeted carbs (if carb day)
• Pre-sleep: Casein protein or bone broth
• Electrolyte timing around workouts

Supplement Stack - Phase 2 (Cycling Protocol)

Week 1 & 3 of Phase 2:

• Continue Phase 1 stack
• Add: Berberine 500mg (with meals on carb days)
• Add: Alpha-GPC 600mg (pre-workout)

Week 2 & 4 of Phase 2:

• Reduce NAD+ precursor to 150mg
• Add: PQQ 20mg
• Add: Urolithin A 500mg
• Cycle off Rhodiola (week 2 only)

New Additions:

• Fisetin: 100mg (2 consecutive days per month)
• Quercetin: 500mg (with zinc 15mg)
• NMN: 250mg (alternate weeks with NR)

Exercise Protocol - Phase 2

Periodization Approach:

• Week 5-6: Strength focus (3-6 rep range)
• Week 7-8: Power and explosiveness

New Additions:

• VO2 Max Training: Weekly 4×4min intervals at 90-95% max HR
• Strength Standards Target:
  • Deadlift: 2× body weight
  • Squat: 1.5× body weight
  • Bench/Push-up equivalent: 1.2× body weight

Weekly Split:

• Monday: Lower Power
• Tuesday: HIIT/VO2 Max
• Wednesday: Upper Strength
• Thursday: Zone 2 + Mobility
• Friday: Full Body Functional
• Saturday: Long Zone 2 (60-90min)
• Sunday: Active recovery

Advanced Stress Resilience

Wim Hof Breathing Protocol:

• 3 rounds daily
• 30-40 deep breaths per round
• Retention holds progressing from 1-3 minutes
• Combine with cold exposure

HRV Training Progression:

• Coherent breathing: 15min daily
• HRV biofeedback training
• Stress inoculation through controlled stressors

Neurofeedback Integration:

• Alpha wave training (8-12 Hz)
• SMR training (12-15 Hz) for focus
• 20min sessions 3×/week

Advanced Tracking

Continuous Glucose Monitor:

• FreeStyle Libre or Dexcom
• Track glucose variability
• Optimize meal timing and composition

Advanced Sleep Tracking:

• Deep sleep percentage target: >20%
• REM sleep target: >20%
• Sleep efficiency: >85%

Cognitive Testing:

• Weekly: Dual N-Back training
• Reaction time tests
• Memory assessments

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Phase 3: Peak Performance (Weeks 9-12)

Precision Nutrition Protocol

Chronobiology-Optimized Eating:

• Largest meal: 12-2pm (circadian peak)
• Minimal evening calories
• Protein timing: 1.6-2.2g/kg body weight
• Strategic carb timing around workouts only

Advanced Fasting Protocols:

• Week 9: Two 36-hour fasts
• Week 10: One 48-hour fast (with medical supervision)
• Week 11-12: 20:4 daily with 2×24-hour weekly

Micronutrient Precision:

• Personalized based on genetic testing (MTHFR, APOE)
• Targeted deficiency correction from lab results
• Anti-inflammatory food focus

Peak Performance Supplement Stack

Nootropic Stack (Training Days):

• Modafinil: 100mg (if legally prescribed)
• Lion's Mane: 1000mg
• Bacopa Monnieri: 600mg
• Phosphatidylserine: 300mg
• Alpha-GPC: 600mg

Longevity Stack (Daily):

• Rapamycin: 5mg weekly (physician supervised)
• Metformin: 500mg (if insulin resistant)
• Spermidine: 1mg daily
• Urolithin A: 500mg
• NAD+ precursor: 500mg

Performance Stack:

• Creatine: 5g daily
• Beta-Alanine: 3g daily
• HMB: 3g daily
• Citrulline Malate: 8g pre-workout

Elite Exercise Protocol

Strength-Power Fusion:

• Complex training (heavy lift + explosive movement)
• Cluster sets for power development
• Accommodating resistance (bands/chains)

Metabolic Conditioning:

• Lactate threshold training
• VO2 max intervals
• Aerobic power development

Recovery Modalities:

• Contrast therapy (hot/cold)
• Compression therapy
• Red light therapy (660-850nm): 20min daily
• Percussive therapy (Theragun)

Advanced Biohacking Technologies

Photobiomodulation:

• Red light panel: 660nm + 850nm
• 20min daily exposure
• Target: mitochondrial optimization

Pulsed Electromagnetic Field (PEMF):

• 8Hz frequency for recovery
• 30min sessions post-workout

Hyperbaric Oxygen Therapy:

• 1.3-1.5 ATA pressure
• 60-90min sessions
• 2-3×/week for recovery enhancement

Stress Mastery & Cognitive Enhancement

Advanced HRV Protocols:

• Real-time HRV feedback during stress
• Progressive stress inoculation
• Target: HRV >50ms (RMSSD)

Meditation Progression:

• 30min daily practice
• Vipassana or concentration techniques
• Neurofeedback-assisted meditation

Cognitive Training:

• Working memory training
• Processing speed enhancement
• Executive function optimization

Comprehensive Testing & Optimization

Week 12 Advanced Panel:

• Comprehensive hormonal assessment
• Advanced lipid analysis (NMR)
• Inflammatory markers
• Oxidative stress markers
• Telomere length testing
• Biological age assessment

Performance Testing:

• VO2 max testing
• Body composition (DEXA scan)
• Cognitive assessment battery
• Strength and power testing
• Flexibility and mobility assessment

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Daily Schedule Template (Phase 3)

6:00 AM: Wake, HRV measurement, morning supplements 6:15 AM: Meditation + breathing exercises 6:45 AM: Red light therapy 7:15 AM: Exercise (fasted) 8:30 AM: Post-workout nutrition 12:00 PM: Main meal (largest) 2:00 PM: Cognitive training/work 6:00 PM: Light meal (if eating day) 7:00 PM: Evening walk + family time 8:00 PM: Sauna or cold exposure 9:00 PM: Blue light blocking, evening supplements 10:00 PM: Sleep preparation 10:30 PM: Sleep

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Safety Considerations & Monitoring

Red Flags to Monitor:

• Persistent HRV decline
• Sleep quality deterioration
• Extreme fatigue or mood changes
• Digestive issues
• Injury or overtraining symptoms

Medical Supervision Required:

• Rapamycin protocols
• Extended fasting (>24 hours)
• Any prescription nootropics
• Hormone optimization

Adjustment Protocols:

• Weekly plan modifications based on biomarkers
• Deload weeks if overreaching detected
• Supplement cycling to prevent tolerance

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Expected Outcomes (3-Month Targets)

Biomarkers:

• Fasting glucose: <90 mg/dL
• HbA1c: <5.4%
• Triglycerides: <100 mg/dL
• HDL: >60 mg/dL
• CRP: <1.0 mg/L
• Resting HR: Decrease 5-10 bpm

Performance:

• VO2 max: Increase 10-15%
• Strength gains: 15-25%
• Body fat: Decrease 3-5%
• Cognitive scores: Improve 10-20%
• HRV: Increase 20-30%

Subjective:

• Energy levels: Sustained throughout day
• Sleep quality: >85% efficiency
• Stress resilience: Improved recovery
• Mental clarity: Enhanced focus and memory

This protocol should be implemented under professional guidance and adjusted based on individual responses a nd biomarker feedback.